Nanomaterial effects on C. elegans fitness

Nanomaterial: Graphene
Concentration: 50 ppm (dispersant: DI water).
Control: DI Water
Date: 2014-04-25, by: SangKyu
Test Method

OD Readings

Avg. OD changes

Day 5
Fitness: 0.36
Day 6
Fitness: 0.383
Day 7
Fitness: 0.779
OD change average S.D. n p value
(Student-t test)
  OD change average S.D. n p value
(Student-t test)
  OD change average S.D. n p value
(Student-t test)
 
Control 0.412 0.099 12 -   Control 1.331 0.133 12 -   Control 1.588 0.082 12 -  
Nanomaterial 0.149 0.02 3 5.8e-8   Nanomaterial 0.51 0.018 3 2.3e-15   Nanomaterial 1.237 0.073 3 3.6e-6  

 

Raw Data

   Well   Day 0   Day 5   Day 6   Day 7 
Control 1 1.7988 1.4544 0.5457 0.2919
2 1.7388 1.4331 0.5715 0.2874
3 1.8321 1.4757 0.585 0.276
4 1.8189 1.497 0.5313 0.2736
5 1.8057 1.4571 0.558 0.2736
6 1.8912 1.5318 0.5976 0.2796
7 1.8939 1.446 0.5409 0.3249
8 2.0337 1.4397 0.327 0.2757
9 1.8801 1.4559 0.5469 0.3015
10 2.0031 1.4121 0.5607 0.2997
11 1.8723 1.5114 0.597 0.2904
12 1.9419 1.407 0.5391 0.2793
Nanomaterial 1 1.8735 1.6836 1.3359 0.4842
2 1.8171 1.6101 1.2456 0.5976
3 1.8495 1.6122 1.2714 0.4962
Control without bacteria 1 0.2589 0.2481 0.2592 0.2586
2 0.2559 0.2517 0.255 0.2556
3 0.2547 0.2517 0.2601 0.2628
4 0.2592 0.2571 0.255 0.2574
5 0.2562 0.2508 0.2508 0.2574
6 0.2589 0.249 0.2454 0.2538
7 0.255 0.2508 0.2526 0.2643
8 0.2571 0.2622 0.2514 0.264
9 0.2571 0.2523 0.2577 0.2529
10 0.2613 0.2682 0.2574 0.2559
11 0.2589 0.258 0.2559 0.2544
12 0.2583 0.2514 0.2523 0.255
Nanomaterial without bacteria 1 0.4392 0.3216 0.3222 0.3045
2 0.3327 0.3441 0.3813 0.3111
3 0.3978 0.3156 0.3087 0.303