Nanomaterial effects on C. elegans fitness

Nanomaterial: Graphene
Concentration: 25 ppm (dispersant: DI water).
Control: DI Water
Date: 2014-04-25, by: SangKyu
Test Method

OD Readings

Avg. OD changes

Day 5
Fitness: 0.433
Day 6
Fitness: 0.516
Day 7
Fitness: 0.887
OD change average S.D. n p value
(Student-t test)
  OD change average S.D. n p value
(Student-t test)
  OD change average S.D. n p value
(Student-t test)
 
Control 0.412 0.099 12 -   Control 1.331 0.133 12 -   Control 1.588 0.082 12 -  
Nanomaterial 0.178 0.014 3 2.0e-7   Nanomaterial 0.687 0.062 3 2.9e-10   Nanomaterial 1.408 0.023 3 3.6e-6  

 

Raw Data

   Well   Day 0   Day 5   Day 6   Day 7 
Control 1 1.7988 1.4544 0.5457 0.2919
2 1.7388 1.4331 0.5715 0.2874
3 1.8321 1.4757 0.585 0.276
4 1.8189 1.497 0.5313 0.2736
5 1.8057 1.4571 0.558 0.2736
6 1.8912 1.5318 0.5976 0.2796
7 1.8939 1.446 0.5409 0.3249
8 2.0337 1.4397 0.327 0.2757
9 1.8801 1.4559 0.5469 0.3015
10 2.0031 1.4121 0.5607 0.2997
11 1.8723 1.5114 0.597 0.2904
12 1.9419 1.407 0.5391 0.2793
Nanomaterial 1 1.8144 1.6086 1.0737 0.3561
2 1.8573 1.6566 1.2213 0.3963
3 1.7853 1.5522 1.0005 0.3735
Control without bacteria 1 0.2589 0.2481 0.2592 0.2586
2 0.2559 0.2517 0.255 0.2556
3 0.2547 0.2517 0.2601 0.2628
4 0.2592 0.2571 0.255 0.2574
5 0.2562 0.2508 0.2508 0.2574
6 0.2589 0.249 0.2454 0.2538
7 0.255 0.2508 0.2526 0.2643
8 0.2571 0.2622 0.2514 0.264
9 0.2571 0.2523 0.2577 0.2529
10 0.2613 0.2682 0.2574 0.2559
11 0.2589 0.258 0.2559 0.2544
12 0.2583 0.2514 0.2523 0.255
Nanomaterial without bacteria 1 0.36 0.2838 0.2829 0.2823
2 0.3045 0.2832 0.2829 0.2841
3 0.2931 0.2862 0.2907 0.285