|Aggregate Size||109.4 ± 7.2 nm |
Toxicity test method
Suspension preparation method
50 mL of the 10 mg/L nC60 suspension was added to each of four 50-mL Erlenmeyer flasks, sealed with parafilm with vents. The four Erlenmeyer flasks were placed on the merry-go round at the center of a luzchem LZC-4V photoreactor (Luzchem, Ottawa, Ontario, Canada), and slowly rotated to ensure uniform exposure to the light source. Four 8W Black-light-lamps (EIKO F8T5/BL, 2 on each side) with an emitting spectrum in the UVA range (centered at 350 nm) provided ~ 2.0 mW/cm2 light intensity as measured by a radiometer (Control Company Friendswood. TX). After 7 days of UVA irradiation, the UV-irradiated nC60 suspension from the four flasks was combined and concentrated to 200 mg/L using 30 kDa MWCO centrifugal concentrators (Amicon Ultra, Millipore, Massachusetts). The sample was stored at room temperature in a 50-mL centrifuge tube wrapped in aluminum foil for up to 1 week before use.
Particle size measurement method
ENM electrophoretic mobility measurement.
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