|Aggregate Size||101.9 ± 40.4 nm |
Toxicity test method
Suspension preparation method
150 mg sublimed C60 powder was added to 200 ml of deionized water (18.2 MΩ cm, 25°C) in a 500 ml glass beaker and sonicated in an ice bath with the Vibra-Cell VCX 500 sonicating probe at 100 W for 30 min. After sonication, the mixture was filtered through 2 µm pore size Millex-AP membrane filters ( Millipore, Billerica, MA) to remove large particles. All filters were washed with 10 mL of DI water before use and replaced after 30 mL of filtrate was collected. The nC60 concentration of the resulting clear yellow suspension was determined by measuring the total organic carbon (TOC) concentration using a high sensitivity TOC analyzer (Shimadzu Scientific Instruments, Columbia, MD). The suspension was concentrated to 200 mg/L using 30 kDa MWCO centrifugal concentrators (Amicon Ultra. Millipore, Massachusetts). The sample was stored at room temperature in a 500 mL clear glass bottle wrapped in aluminum foil before use within 6 months.
Particle size measurement method
ENM electrophoretic mobility measurement.
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